Human H2AB1 Recombinant Protein (C-His) (HY202012)

Name: Human H2AB1 Recombinant Protein (C-His)
Brand: abinScience
SKU: HY202012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HY202012
Description	Recombinant Human H2AB1 Protein, C-His (HY202012) expressed in E. coli, spanning Met1-Asp115. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	P0C5Y9
Longueur de protéine	Met1-Asp115
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	13.76 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Histone H2A-Bbd type 1, H2A Barr body-deficient, H2A.B, H2A.Bbd, H2AB1, H2AFB1
Contexte	Histone H2A-Bbd type 1 (H2AB1) is a ~12 kDa protein. Atypical histone H2A which can replace conventional H2A in some nucleosomes and is associated with active transcription and mRNA processing. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Nucleosomes containing this histone are less rigid and organize less DNA than canonical nucleosomes in vivo. They are enriched in actively transcribed genes and associate with the elongating form of RNA polymerase. 1. Tolstorukov, MY. et al. (2012) Molecular cell 47, 596-607. PMID: 22795134 2. Bao, Y. et al. (2004) The EMBO journal 23, 3314-24. PMID: 15257289 3. Okuwaki, M. et al. (2005) Molecular and cellular biology 25, 10639-51. PMID: 16287874 4. Doyen, CM. et al. (2006) The EMBO journal 25, 4234-44. PMID: 16957777 5. Menoni, H. et al. (2007) Molecular and cellular biology 27, 5949-56. PMID: 17591702 6. Eirín-López, JM. et al. (2008) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 22, 316-26. PMID: 17726088 7. González-Romero, R. et al. (2008) Gene 413, 1-7. PMID: 18329190
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human H2AB1 Protein, C-His

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Human H2AB1 Recombinant Protein (C-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

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g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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