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Human HADHB Recombinant Protein (N-His) (HD094012)

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Vista general
Número de catálogoHD094012
Descripción
Recombinant Human HADHB Protein, N-His (HD094012) expressed in E. coli, spanning Asn50-Ser284. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresiónE. coli
Número de accesoP55084
Longitud de proteínaAsn50-Ser284
AplicacionesELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspeciesHomo sapiens (Human)
Naturaleza Recombinant
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >90% as determined by SDS-PAGE.
Peso molecular predicho 27.59 kDa
Forma Lyophilized
Buffer de almacenamiento Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Reconstitución Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Envío In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos3-ketoacyl-CoA thiolase, Acetyl-CoA acyltransferase, Beta-ketothiolase, EC:2.3.1.155, EC:2.3.1.16, HADHB, TP-beta, Trifunctional enzyme subunit beta, mitochondrial
Fondo

Trifunctional enzyme subunit beta, mitochondrial is a ~51 kDa protein. Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities, while the trifunctional enzyme subunit beta/HADHB described here bears the 3-ketoacyl-CoA thiolase activity.

1. Liang, K. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 7039-7044. PMID: 29915090
2. Xia, C. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 6069-6074. PMID: 30850536
3. Kamijo, T. et al. (1994) Biochemical and biophysical research communications 199, 818-25. PMID: 8135828
Nota For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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