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| カタログ番号 | HC626022 |
|---|---|
| 説明 |
Recombinant Human HDAC1 Protein, N-His (HC626022) expressed in E. coli, spanning Met1-Ala482. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q13547 |
| タンパク質長 | Met1-Ala482 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 57.26 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Histone deacetylase 1, HDAC1, RPD3L1, HD1 |
| 背景 | Histone deacetylase 1 (HDAC1) is a ~55 kDa protein. Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. As part of the SIN3B complex is recruited downstream of the constitutively active genes transcriptional start sites through interaction with histones and mitigates histone acetylation and RNA polymerase II progression within transcribed regions contributing to the regulation of transcription. 1. Qiu, Y. et al. (2006) Molecular cell 22, 669-79. PMID: 16762839 2. Wang, C. et al. (2007) The Journal of biological chemistry 282, 29902-9. PMID: 17704056 3. Wei, W. et al. (2017) Cell research 27, 898-915. PMID: 28497810 4. Le Guezennec, X. et al. (2006) Molecular and cellular biology 26, 843-51. PMID: 16428440 5. Hoffmeister, H. et al. (2017) Nucleic acids research 45, 10534-10554. PMID: 28977666 6. Jelinic, P. et al. (2011) Molecular and cellular biology 31, 54-62. PMID: 21041482 8. Ray, S. et al. (2005) Gastroenterology 129, 1616-32. PMID: 16285960 9. Zheng, G. et al. (2006) Biochemical and biophysical research communications 339, 1069-75. PMID: 16337145 10. Hung, JJ. et al. (2006) Molecular and cellular biology 26, 1770-85. PMID: 16478997 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human HDAC1 Protein, N-His
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