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| カタログ番号 | HT281012 |
|---|---|
| 説明 |
Recombinant Human HDAC3 Protein, N-His (HT281012) expressed in E. coli, spanning Met1-Gln376. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | O15379 |
| タンパク質長 | Met1-Gln376 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 45.03 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | HD3, SMAP45, RPD3-2, Histone deacetylase 3, HDAC3 |
| 背景 | Histone deacetylase 3 (HDAC3) is a ~48 kDa protein. Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes, such as N-Cor repressor complex, which activate the histone deacetylase activity. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation. 1. Chini, CC. et al. (2010) The Journal of biological chemistry 285, 40830-7. PMID: 21030595 2. Pillai, VB. et al. (2011) Molecular and cellular biology 31, 2349-63. PMID: 21444723 3. Hatzi, K. et al. (2013) Cell reports 4, 578-88. PMID: 23911289 4. Yi, J. et al. (2014) The Journal of biological chemistry 289, 33878-86. PMID: 25301942 5. Lee, DY. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, 2072-2077. PMID: 28167758 6. Wei, W. et al. (2017) Cell research 27, 898-915. PMID: 28497810 7. Zhan, W. et al. (2020) Oncogenesis 9, 48. PMID: 32404892 8. Watson, PJ. et al. (2012) Nature 481, 335-40. PMID: 22230954 10. Yuan, ZL. et al. (2005) Science (New York, N.Y.) 307, 269-73. PMID: 15653507 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human HDAC3 Protein, N-His
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