中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HA105012 |
|---|---|
| 説明 |
Recombinant Human Histone H1.2 Protein, N-GST & C-His (HA105012) expressed in E. coli, spanning Ser41-Asn108. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P16403 |
| タンパク質長 | Ser41-Asn108 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 35.12 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | H1-2, H1F2, HIST1H1C, Histone H1.2, Histone H1c, Histone H1d, Histone H1s-1 |
| 背景 | Histone H1.2 is a ~21 kDa protein. Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histone H1-2 is required for the condensation of nucleosome chains into higher-order structured fibers. Compared to other histone H1 variants, H1-2 plays an essnetial role in nucleosome condensation: its absence leads to global chromatin decompaction, which is not observed when depleting other histone H1 variants. Histone H1-2 also acts as a histone reader: specifically recognizes and binds histone H3 trimethylated at 'lys-27' (H3K27me3). Histones H1 also promote formation of the H3K27me3 mark by the PRC2/EED-EZH2 complex, possibly by facilitating restoration of H3K27me3 post-replication. 1. Kim, JM. et al. (2015) Scientific reports 5, 16714. PMID: 26581166 2. Salinas-Pena, M. et al. (2024) eLife 12. PMID: 38530350 3. Liu, C. et al. (2023) Nature communications 14, 4081. PMID: 37429872 4. Matthews, RE. et al. (2025) Molecular cell 85, 2503-2516.e8. PMID: 40516528 5. Pascal, C. et al. (2023) Molecular cell 83, 3801-3817.e8. PMID: 37922872 |
| 注意事項 | For research use only |
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
