Human HLA-A Recombinant Protein (N-His) (HM834012)

Name: Human HLA-A Recombinant Protein (N-His)
Brand: abinScience
SKU: HM834012
Price: 211 USD
Availability: InStock

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1mg

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号	HM834012
説明	Recombinant Human HLA-A Protein, N-His (HM834012) expressed in E. coli, spanning Gly25-Val307. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
発現システム	E. coli
アクセッション番号	P04439
タンパク質長	Gly25-Val307
アプリケーション	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
種	Homo sapiens (Human)
性質	Recombinant
エンドトキシンレベル	Please contact with the lab for this information.
純度	>90% as determined by SDS-PAGE.
予測分子量	34.48 kDa
形態	Lyophilized
保存バッファー	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
再構成	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
発送	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名	HLAA, Human leukocyte antigen A, HLA class I histocompatibility antigen, A alpha chain, HLA-A
背景	HLA class I histocompatibility antigen, A alpha chain (HLA-A) is a ~40 kDa protein. Antigen-presenting major histocompatibility complex class I (MHCI) molecule. In complex with B2M/beta 2 microglobulin displays primarily viral and tumor-derived peptides on antigen-presenting cells for recognition by alpha-beta T cell receptor (TCR) on HLA-A-restricted CD8-positive T cells, guiding antigen-specific T cell immune response to eliminate infected or transformed cells. May also present self-peptides derived from the signal sequence of secreted or membrane proteins, although T cells specific for these peptides are usually inactivated to prevent autoreactivity. Both the peptide and the MHC molecule are recognized by TCR, the peptide is responsible for the fine specificity of antigen recognition and MHC residues account for the MHC restriction of T cells. Typically presents intracellular peptide antigens of 8 to 13 amino acids that arise from cytosolic proteolysis via IFNG-induced immunoproteasome or via endopeptidase IDE/insulin-degrading enzyme. 1. Fukada, K. et al. (1999) AIDS (London, England) 13, 1413-4. PMID: 10449296 2. Nagata, Y. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 10629-34. PMID: 12138174 3. Kuzushima, K. et al. (2003) Blood 101, 1460-8. PMID: 12393434 4. Traversari, C. et al. (1992) The Journal of experimental medicine 176, 1453-7. PMID: 1402688 5. Satoh, M. et al. (2005) Vaccine 23, 3783-90. PMID: 15893615 6. Asemissen, AM. et al. (2006) Clinical cancer research : an official journal of the American Association for Cancer Research 12, 7476-82. PMID: 17189421 7. Hadrup, SR. et al. (2009) Nature methods 6, 520-6. PMID: 19543285 8. Matthews, PC. et al. (2011) Journal of immunology (Baltimore, Md. : 1950) 186, 5675-86. PMID: 21498667 9. Shimizu, A. et al. (2013) Scientific reports 3, 3097. PMID: 24192765 10. Quiñones-Parra, S. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 1049-54. PMID: 24395804
注意事項	For research use only.