Human HNRNPA1 Recombinant Protein (N-His) (HY212012)

Name: Human HNRNPA1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HY212012
Price: 211 USD
Availability: InStock

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1mg

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SDS

Vista general

Número de catálogo	HY212012
Descripción	Recombinant Human HNRNPA1 Protein, N-His (HY212012) expressed in E. coli, spanning Ser2-Phe372. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P09651
Longitud de proteína	Ser2-Phe372
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	40.91 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	HNRNPA1, Single-strand RNA-binding protein, Heterogeneous nuclear ribonucleoprotein A1, hnRNP A1, hnRNP core protein A1, Helix-destabilizing protein, HNRPA1
Fondo	Heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1) is a ~38 kDa protein. Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and modulation of splice site selection. Plays a role in the splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform. Binds to the IRES and thereby inhibits the translation of the apoptosis protease activating factor APAF1. May bind to specific miRNA hairpins. 1. Paronetto, MP. et al. (2007) The Journal of cell biology 176, 929-39. PMID: 17371836 2. David, CJ. et al. (2010) Nature 463, 364-8. PMID: 20010808 3. Li, ML. et al. (2019) PloS one 14, e0221048. PMID: 31498791 4. Treiber, T. et al. (2017) Molecular cell 66, 270-284.e13. PMID: 28431233
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Human HNRNPA1 Recombinant Protein (N-His)

Referencias

Datasheet

Human HNRNPA1 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

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g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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