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| カタログ番号 | HX893012 |
|---|---|
| 説明 |
Recombinant Human HNRNPU Protein, N-His (HX893012) expressed in E. coli, spanning Asp296-Lys462. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q00839 |
| タンパク質長 | Asp296-Lys462 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 21.11 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | C1orf199, GRIP120, pp120, Scaffold-attachment factor A, p120, SAFA, Nuclear p120 ribonucleoprotein, hnRNP U, SAF-A, Heterogeneous nuclear ribonucleoprotein U, HNRNPU, HNRPU, U21.1 |
| 背景 | Heterogeneous nuclear ribonucleoprotein U (HNRNPU) is a ~90 kDa protein. DNA- and RNA-binding protein involved in several cellular processes such as nuclear chromatin organization, telomere-length regulation, transcription, mRNA alternative splicing and stability, Xist-mediated transcriptional silencing and mitotic cell progression. Plays a role in the regulation of interphase large-scale gene-rich chromatin organization through chromatin-associated RNAs (caRNAs) in a transcription-dependent manner, and thereby maintains genomic stability. Required for the localization of the long non-coding Xist RNA on the inactive chromosome X (Xi) and the subsequent initiation and maintenance of X-linked transcriptional gene silencing during X-inactivation. Plays a role as a RNA polymerase II (Pol II) holoenzyme transcription regulator. Promotes transcription initiation by direct association with the core-TFIIH basal transcription factor complex for the assembly of a functional pre-initiation complex with Pol II in a actin-dependent manner. 1. Kim, MK. et al. (1999) Molecular and cellular biology 19, 6833-44. PMID: 10490622 2. Fu, D. et al. (2007) Molecular cell 28, 773-85. PMID: 18082603 3. Weidensdorfer, D. et al. (2009) RNA (New York, N.Y.) 15, 104-15. PMID: 19029303 4. Xiao, R. et al. (2012) Molecular cell 45, 656-68. PMID: 22325991 5. Douglas, P. et al. (2015) Molecular and cellular biology 35, 2699-713. PMID: 25986610 6. Nozawa, RS. et al. (2017) Cell 169, 1214-1227.e18. PMID: 28622508 7. Romig, H. et al. (1992) The EMBO journal 11, 3431-40. PMID: 1324173 8. Fackelmayer, FO. et al. (1994) European journal of biochemistry 221, 749-57. PMID: 8174554 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human HNRNPU Protein, N-His
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