Human HSPA8 Recombinant Protein (N-His) (HY600012)

Name: Human HSPA8 Recombinant Protein (N-His)
Brand: abinScience
SKU: HY600012
Price: 211 USD
Availability: InStock

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1mg

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号	HY600012
説明	Recombinant Human HSPA8 Protein, N-His (HY600012) expressed in E. coli, spanning Asp534-Gly615. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
発現システム	E. coli
アクセッション番号	P11142
タンパク質長	Asp534-Gly615
アプリケーション	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
種	Homo sapiens (Human)
性質	Recombinant
エンドトキシンレベル	Please contact with the lab for this information.
純度	>90% as determined by SDS-PAGE.
予測分子量	11.83 kDa
形態	Lyophilized
保存バッファー	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
再構成	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
発送	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名	Lipopolysaccharide-associated protein 1, HSPA10, Heat shock 70 kDa protein 8, LPS-associated protein 1, LAP-1, HSP73, HSC70, HSPA8, Heat shock cognate 71 kDa protein
背景	Heat shock cognate 71 kDa protein (HSPA8) is a ~70 kDa protein. Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, chaperone-mediated autophagy, activation of proteolysis of misfolded proteins, formation and dissociation of protein complexes, and antigen presentation. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The co-chaperones have been shown to not only regulate different steps of the ATPase cycle of HSP70, but they also have an individual specificity such that one co-chaperone may promote folding of a substrate while another may promote degradation. The affinity of HSP70 for polypeptides is regulated by its nucleotide bound state. 1. Grove, DE. et al. (2011) Molecular biology of the cell 22, 301-14. PMID: 21148293 2. Yamamoto, YH. et al. (2010) Cell structure and function 35, 107-16. PMID: 21150129 3. Sopha, P. et al. (2012) Cell structure and function 37, 177-87. PMID: 23018488 4. Goodwin, EC. et al. (2014) PloS one 9, e94322. PMID: 24732912 5. Li, K. et al. (2017) Molecular cell 65, 52-65. PMID: 27916661 6. Chiang, HL. et al. (1989) Science (New York, N.Y.) 246, 382-5. PMID: 2799391 7. Wang, L. et al. (2023) Molecular cell 83, 281-297.e10. PMID: 36586411 8. Young, JC. et al. (2003) Cell 112, 41-50. PMID: 12526792 10. Rauch, JN. et al. (2014) The Journal of biological chemistry 289, 1402-14. PMID: 24318877
注意事項	For research use only.