ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces
Homo sapiens (Human)
Nature
Recombinant
Niveau d'endotoxines
Please contact with the lab for this information.
Pureté
>90% as determined by SDS-PAGE.
Forme
Lyophilized
Tampon de stockage
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Expédition
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs
ARF-BP1, ARF-binding protein 1, E3 ubiquitin-protein ligase HUWE1, EC:2.3.2.26, HECT, UBA and WWE domain-containing protein 1, HECT-type E3 ubiquitin transferase HUWE1, HUWE1, HectH9, Homologous to E6AP carboxyl terminus homologous protein 9, KIAA0312, KIAA1578, LASU1, Large structure of UREB1, Mcl-1 ubiquitin ligase E3, Mule, URE-B1, URE-binding protein 1, UREB1, Upstream regulatory element-binding protein 1
Contexte
E3 ubiquitin-protein ligase HUWE1 is a ~481 kDa protein. E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Ubiquitinates MFN2 to negatively regulate mitochondrial fusion in response to decreased stearoylation of TFRC.
1. Yoon, SY. et al. (2005) Biochemical and biophysical research communications 326, 7-17. PMID: 15567145
2. Liu, Z. et al. (2005) Molecular and cellular biology 25, 2819-31. PMID: 15767685
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