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| カタログ番号 | HD965012 |
|---|---|
| 説明 |
Recombinant Human HUWE1 Protein, N-His (HD965012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q7Z6Z7 |
| タンパク質長 | Leu4005-Ala4374 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | ARF-BP1, ARF-binding protein 1, E3 ubiquitin-protein ligase HUWE1, EC:2.3.2.26, HECT, UBA and WWE domain-containing protein 1, HECT-type E3 ubiquitin transferase HUWE1, HUWE1, HectH9, Homologous to E6AP carboxyl terminus homologous protein 9, KIAA0312, KIAA1578, LASU1, Large structure of UREB1, Mcl-1 ubiquitin ligase E3, Mule, URE-B1, URE-binding protein 1, UREB1, Upstream regulatory element-binding protein 1 |
| 背景 | E3 ubiquitin-protein ligase HUWE1 is a ~481 kDa protein. E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Ubiquitinates MFN2 to negatively regulate mitochondrial fusion in response to decreased stearoylation of TFRC. 1. Yoon, SY. et al. (2005) Biochemical and biophysical research communications 326, 7-17. PMID: 15567145 2. Liu, Z. et al. (2005) Molecular and cellular biology 25, 2819-31. PMID: 15767685 3. Zhong, Q. et al. (2005) Cell 121, 1085-95. PMID: 15989957 4. Hall, JR. et al. (2007) Molecular biology of the cell 18, 3340-50. PMID: 17567951 5. Zhao, X. et al. (2008) Nature cell biology 10, 643-53. PMID: 18488021 6. Liu, Z. et al. (2009) Molecular biology of the cell 20, 757-68. PMID: 19037095 7. Parsons, JL. et al. (2009) The EMBO journal 28, 3207-15. PMID: 19713937 8. Yin, L. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 11614-9. PMID: 20534529 9. Di Rita, A. et al. (2018) Nature communications 9, 3755. PMID: 30217973 |
| 注意事項 | For research use only |
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