Human IFN-gamma Recombinant Protein (N-His) (HF813012)

Name: Human IFN-gamma Recombinant Protein (N-His)
Brand: abinScience
SKU: HF813012
Price: 211 USD
Availability: InStock

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Vista general

Número de catálogo	HF813012
Descripción	Recombinant Human IFNG/IFN-gamma Protein, N-His (HF813012) expressed in E. coli, spanning Gln24-Gly161. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P01579
Longitud de proteína	Gln24-Gly161
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Aplicaciones probadas	WB
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	18.47 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Interferon gamma, IFN-gamma, Immune interferon, IFNG
Fondo	Interferon gamma (IFNG/IFN-gamma) is a ~19 kDa protein. Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. 1. El Bougrini, J. et al. (2006) Cellular and molecular biology (Noisy-le-Grand, France) 52, 9-15. PMID: 16914093 2. Hisamatsu, H. et al. (1996) The Journal of experimental medicine 183, 1807-16. PMID: 8666937 3. Greenlund, AC. et al. (1993) The Journal of biological chemistry 268, 18103-10. PMID: 8349687 4. Akiyama, K. et al. (1994) FEBS letters 343, 85-8. PMID: 8163024 5. Fabunmi, RP. et al. (2001) Journal of cell science 114, 29-36. PMID: 11112687 6. Lah, TT. et al. (1995) FEBS letters 363, 85-9. PMID: 7729559
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human IFNG/IFN-gamma Protein, N-His
Western blot

Detected by Anti-IFNG/IFN-gamma monoclonal antibody (Catalog No: HY328043)
Bioactivity

Detected by SAA1294 (Catalog No: HF813023) in indirect ELISA.
Bioactivity

Detected by AMG 811 (Catalog No: HF813036) in indirect ELISA.