Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Interleukin-10 (IL10) is a ~20 kDa protein. Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF. Also interferes with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation.
1. Yoon, SI. et al. (2006) The Journal of biological chemistry 281, 35088-96. PMID: 16982608
2. El Kasmi, KC. et al. (2007) Journal of immunology (Baltimore, Md. : 1950) 179, 7215-9. PMID: 18025162
3. Hashimoto, SI. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 167, 3619-25. PMID: 11564774
4. de Waal Malefyt, R. et al. (1991) The Journal of experimental medicine 174, 1209-20. PMID: 1940799
5. Willems, F. et al. (1994) European journal of immunology 24, 1007-9. PMID: 7512027
6. Frei, K. et al. (1994) Journal of immunology (Baltimore, Md. : 1950) 152, 2720-8. PMID: 8144879
Примечание
For research use only.
Изображения
SDS-PAGE
SDS-PAGE for Recombinant Human IL10 Protein, C-His
Bioactivity
Immobilized HB997011 Human IL10 Recombinant Protein (C-His) at 2 μg/mL(100 μL/well) can bind HC614021 Human CDw210a/IL10RA Recombinant Protein (C-Fc), the EC 50 is 325 ng/mL.
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