Human IL-18 Recombinant Protein (C-His) (HC517012)

Name: Human IL-18 Recombinant Protein (C-His)
Brand: abinScience
SKU: HC517012
Price: 211 USD
Availability: InStock

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1mg

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Vista general

Número de catálogo	HC517012
Descripción	Recombinant Human IL18/IL1F4 Protein, C-His (HC517012) expressed in E. coli, spanning Tyr37-Asp193. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q14116
Longitud de proteína	Tyr37-Asp193
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Aplicaciones probadas	WB
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	19.47 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	IFN-gamma-inducing factor, IL1F4, Interleukin-1 gamma, IL-18, IL-1 gamma, Interleukin-18, Interferon gamma-inducing factor, IGIF, IL18, Iboctadekin
Fondo	Interleukin-18 (IL18/IL1F4) is a ~22 kDa protein. Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. 1. Tominaga, K. et al. (2000) International immunology 12, 151-60. PMID: 10653850 2. Kato, Z. et al. (2003) Nature structural biology 10, 966-71. PMID: 14528293 3. Tsutsumi, N. et al. (2014) Nature communications 5, 5340. PMID: 25500532 4. Shi, X. et al. (2023) Nature 624, 442-450. PMID: 37993714 5. Xia, S. et al. (2021) Nature 593, 607-611. PMID: 33883744
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human IL18/IL1F4 Protein, C-His
Western blot

Detected by Anti-IL18/IL1F4 monoclonal antibody (Catalog No: HC517046)
Bioactivity

Detected by Research Grade Anti-Human IL18/IL1F4 (GSK 1070806) (Catalog No: HC517046) in indirect ELISA