Human IL7 Recombinant Protein (C-His) (HY408012)

Name: Human IL7 Recombinant Protein (C-His)
Brand: abinScience
SKU: HY408012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Обзор

Каталожный номер	HY408012
Описание	Recombinant Human IL7 Protein, C-His (HY408012) expressed in E. coli, spanning Asp26-His177. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	P13232
Длина белка	Asp26-His177
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	18.64 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	IL-7, Interleukin-7, IL7
Фон	Interleukin-7 (IL7) is a ~20 kDa protein. Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maintaining lymphoid homeostasis. Mechanistically, exerts its biological effects through a receptor composed of IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG. Binding to the receptor leads to activation of various kinases including JAK1 or JAK3 depending on the cell type and subsequently propagation of signals through activation of several downstream signaling pathways including the PI3K/Akt/mTOR or the JAK-STAT5. 1. Bazdar, DA. et al. (2015) Journal of immunology (Baltimore, Md. : 1950) 194, 4698-704. PMID: 25870237 2. Dittel, BN. et al. (1995) Journal of immunology (Baltimore, Md. : 1950) 154, 58-67. PMID: 7527823 3. Kondo, M. et al. (1994) Science (New York, N.Y.) 263, 1453-4. PMID: 8128231 4. Johnson, SE. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 180, 8109-17. PMID: 18523275 5. Rochman, Y. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 19455-60. PMID: 20974963
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human IL7 Protein, C-His

Ссылки

Даташит

Human IL7 Recombinant Protein (C-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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