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| カタログ番号 | HD813012 |
|---|---|
| 説明 |
Recombinant Human INSIG2 Protein, N-GST & C-His (HD813012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9Y5U4 |
| タンパク質長 | Ala2-Leu28&Gln52-Ala70&Pro89-Arg103&Arg208-Glu225 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | INSIG-2, INSIG2, Insulin-induced gene 2 protein |
| 背景 | Insulin-induced gene 2 protein is a ~24 kDa protein. Oxysterol-binding protein that mediates feedback control of cholesterol synthesis by controlling both endoplasmic reticulum to Golgi transport of SCAP and degradation of HMGCR. Acts as a negative regulator of cholesterol biosynthesis by mediating the retention of the SCAP-SREBP complex in the endoplasmic reticulum, thereby blocking the processing of sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2. Binds oxysterol, including 22-hydroxycholesterol, 24-hydroxycholesterol, 25-hydroxycholesterol and 27-hydroxycholesterol, regulating interaction with SCAP and retention of the SCAP-SREBP complex in the endoplasmic reticulum. In presence of oxysterol, interacts with SCAP, retaining the SCAP-SREBP complex in the endoplasmic reticulum, thereby preventing SCAP from escorting SREBF1/SREBP1 and SREBF2/SREBP2 to the Golgi. Sterol deprivation or phosphorylation by PCK1 reduce oxysterol-binding, disrupting the interaction between INSIG2 and SCAP, thereby promoting Golgi transport of the SCAP-SREBP complex, followed by processing and nuclear translocation of SREBF1/SREBP1 and SREBF2/SREBP2. 1. Yabe, D. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 12753-8. PMID: 12242332 2. Gong, Y. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 6154-9. PMID: 16606821 3. Xu, D. et al. (2020) Nature 580, 530-535. PMID: 32322062 4. Radhakrishnan, A. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 6511-8. PMID: 17428920 5. Ren, R. et al. (2015) Science (New York, N.Y.) 349, 187-91. PMID: 26160948 |
| 注意事項 | For research use only |
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