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| カタログ番号 | HA984012 |
|---|---|
| 説明 |
Recombinant Human INTS8 Protein, N-His (HA984012) expressed in E. coli, spanning Asn831-Phe995. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q75QN2 |
| タンパク質長 | Asn831-Phe995 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 21.55 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Integrator complex subunit 8, Int8, Protein kaonashi-1, C8orf52, INTS8 |
| 背景 | Integrator complex subunit 8 (INTS8) is a ~113 kDa protein. Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes. The integrator complex provides a quality checkpoint during transcription elongation by driving premature transcription termination of transcripts that are unfavorably configured for transcriptional elongation: the complex terminates transcription by (1) catalyzing dephosphorylation of the C-terminal domain (CTD) of Pol II subunit POLR2A/RPB1 and SUPT5H/SPT5, (2) degrading the exiting nascent RNA transcript via endonuclease activity and (3) promoting the release of Pol II from bound DNA. The integrator complex is also involved in terminating the synthesis of non-coding Pol II transcripts, such as enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs and long non-coding RNAs (lncRNAs). Within the integrator complex, INTS8 is required for the recruitment of protein phosphatase 2A (PP2A) to transcription pause-release checkpoint. 1. Oegema, R. et al. (2017) PLoS genetics 13, e1006809. PMID: 28542170 2. Zheng, H. et al. (2020) Science (New York, N.Y.) 370. PMID: 33243860 3. Vervoort, SJ. et al. (2021) Cell 184, 3143-3162.e32. PMID: 34004147 4. Hu, S. et al. (2023) Molecular cell 83, 1588-1604.e5. PMID: 37080207 5. Fianu, I. et al. (2024) Nature 629, 219-227. PMID: 38570683 6. Baillat, D. et al. (2005) Cell 123, 265-76. PMID: 16239144 7. Huang, KL. et al. (2020) Molecular cell 80, 345-358.e9. PMID: 32966759 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human INTS8 Protein, N-His
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