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| カタログ番号 | HC458012 |
|---|---|
| 説明 |
Recombinant Human IRF3 Protein, N-His (HC458012) expressed in E. coli, spanning Met1-Ser123. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q14653 |
| タンパク質長 | Met1-Ser123 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 16.29 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | IRF-3, IRF3, Interferon regulatory factor 3 |
| 背景 | Interferon regulatory factor 3 (IRF3) is a ~47 kDa protein. Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. 1. Tanaka, Y. et al. (2012) Science signaling 5, ra20. PMID: 22394562 2. Wang, S. et al. (2013) Journal of virology 87, 12814-27. PMID: 24049179 3. Liu, S. et al. (2015) Science (New York, N.Y.) 347, aaa2630. PMID: 25636800 4. Zhao, B. et al. (2016) Proceedings of the National Academy of Sciences of the United States of America 113, E3403-12. PMID: 27302953 5. Li, D. et al. (2019) Journal of cell science 133. PMID: 31340999 6. Yang, S. et al. (2023) Molecular cell 83, 298-313.e8. PMID: 36603579 7. Au, WC. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 11657-61. PMID: 8524823 8. Zhu, H. et al. (2024) Cell death & disease 15, 727. PMID: 39362857 9. Yoneyama, M. et al. (2002) Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 22, 73-6. PMID: 11846977 10. Solis, M. et al. (2006) Biochemical pharmacology 72, 1469-76. PMID: 16846591 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human IRF3 Protein, N-His
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