Human KCNK2 Recombinant Protein (N-His) (HF823012)

Name: Human KCNK2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HF823012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Каталожный номер	HF823012
Описание	Recombinant Human KCNK2 Protein, N-His (HF823012) expressed in E. coli, spanning Asp309-Lys426. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	O95069
Длина белка	Asp309-Lys426
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	15.81 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	Potassium channel subfamily K member 2, Outward rectifying potassium channel protein TREK-1, TREK-1 K(+) channel subunit, Two pore domain potassium channel TREK-1, Two pore potassium channel TPKC1, KCNK2, TREK, TREK1
Фон	Potassium channel subfamily K member 2 (KCNK2) is a ~47 kDa protein. K(+) channel that conducts voltage-dependent outward rectifying currents upon membrane depolarization. Voltage sensing is coupled to K(+) electrochemical gradient in an 'ion flux gating' mode where outward but not inward ion flow opens the gate. Converts to voltage-independent 'leak' conductance mode upon stimulation by various stimuli including mechanical membrane stretch, acidic pH, heat and lipids. Reversibly converts between a voltage-insensitive K(+) 'leak' channel and a voltage-dependent outward rectifying K(+) channel in a phosphorylation-dependent manner. Homo- and heterodimerizes to form functional channels with distinct regulatory and gating properties. 1. Patel, AJ. et al. (1999) Nature neuroscience 2, 422-6. PMID: 10321245 2. Meadows, HJ. et al. (2000) Pflugers Archiv : European journal of physiology 439, 714-22. PMID: 10784345 3. Bockenhauer, D. et al. (2001) Nature neuroscience 4, 486-91. PMID: 11319556 4. Plant, LD. et al. (2012) Science signaling 5, ra84. PMID: 23169818 5. Royal, P. et al. (2019) Neuron 101, 232-245.e6. PMID: 30573346 6. Sorum, B. et al. (2024) Nature communications 15, 3142. PMID: 38605031
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human KCNK2 Protein, N-His

Ссылки

Даташит

Human KCNK2 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ (Stock Conc.)

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C₂ (Working Conc.)

V₂ (Working Vol.)

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