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| カタログ番号 | HW621012 |
|---|---|
| 説明 |
Recombinant Human Ki67/MKI67 Protein, N-His (HW621012) expressed in E. coli, spanning Gly3088-Lys3235. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P46013 |
| タンパク質長 | Gly3088-Lys3235 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 18.5 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | MKI67, Proliferation marker protein Ki-67, Antigen Ki67, Antigen KI-67, Antigen identified by monoclonal antibody Ki-67 |
| 背景 | Proliferation marker protein Ki-67 (Ki67/MKI67) is a ~358 kDa protein. Protein that associates with the surface of mitotic chromosomes and acts both as a chromosome repellent during early mitosis and chromosome attractant during late mitosis. Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. During early mitosis, relocalizes from nucleoli to the chromosome surface where it forms extended brush structures that cover a substantial fraction of the chromosome surface. The MKI67 brush structure prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. During mitotic anaphase, the MKI67 brush structure collapses and MKI67 switches from a chromosome repellent to a chromosome attractant to promote chromosome clustering and facilitate the exclusion of large cytoplasmic particles from the future nuclear space. 1. Cuylen, S. et al. (2016) Nature 535, 308-12. PMID: 27362226 2. Cuylen-Haering, S. et al. (2020) Nature 587, 285-290. PMID: 32879492 3. Yamazaki, H. et al. (2022) Nature cell biology 24, 625-632. PMID: 35513709 4. Hernandez-Armendariz, A. et al. (2024) Molecular cell 84, 3254-3270.e9. PMID: 39153474 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human Ki67/MKI67 Protein, N-His
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
