Human KPNA2 Recombinant Protein (N-His) (HW788012)

Name: Human KPNA2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HW788012
Price: 211 USD
Availability: InStock

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Numéro de catalogue	HW788012
Description	Recombinant Human KPNA2 Protein, N-His (HW788012) expressed in E. coli, spanning Ala157-Gln412. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	P52292
Longueur de protéine	Ala157-Gln412
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	29.58 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	SRP1-alpha, Karyopherin subunit alpha-2, RAG cohort protein 1, RCH1, Importin subunit alpha-1, KPNA2, SRP1
Contexte	Importin subunit alpha-1 (KPNA2) is a ~57 kDa protein. Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. 1. Zheng, W. et al. (2018) Traffic (Copenhagen, Denmark) 19, 19-28. PMID: 28991411 2. Cai, Z. et al. (2020) The Journal of experimental medicine 217. PMID: 32130408 3. Moroianu, J. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 6532-6. PMID: 7604027 4. Weis, K. et al. (1995) Science (New York, N.Y.) 268, 1049-53. PMID: 7754385 5. Yang, TJ. et al. (2022) The Journal of cell biology 221. PMID: 35446349
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human KPNA2 Protein, N-His

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Human KPNA2 Recombinant Protein (N-His).pdf

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Formula

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