Human LAPTM4B Recombinant Protein (N-GST & C-His) (HC812012)

Name: Human LAPTM4B Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HC812012
Price: 211 USD
Availability: InStock

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Número de catálogo	HC812012
Descripción	Recombinant Human LAPTM4B Protein, N-GST & C-His (HC812012) expressed in E. coli, spanning Thr2-Thr116. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q86VI4
Longitud de proteína	Thr2-Thr116
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	40.65 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	LAPTM4B, Lysosomal-associated transmembrane protein 4B, Lysosome-associated transmembrane protein 4-beta
Fondo	Lysosomal-associated transmembrane protein 4B is a ~35 kDa protein. Required for optimal lysosomal function. Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation. Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation. Plays a role as negative regulator of TGFB1 production in regulatory T cells. 1. Vergarajauregui, S. et al. (2011) Journal of cell science 124, 459-68. PMID: 21224396 2. Tan, X. et al. (2015) The EMBO journal 34, 475-90. PMID: 25588945 3. Milkereit, R. et al. (2015) Nature communications 6, 7250. PMID: 25998567 4. Huygens, C. et al. (2015) The Journal of biological chemistry 290, 20105-16. PMID: 26126825 5. Blom, T. et al. (2015) Nature chemical biology 11, 799-806. PMID: 26280656
Nota	For research use only

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Datasheet

Human LAPTM4B Recombinant Protein (N-GST & C-His).pdf

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