中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HC812012 |
|---|---|
| 説明 |
Recombinant Human LAPTM4B Protein, N-GST & C-His (HC812012) expressed in E. coli, spanning Thr2-Thr116. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q86VI4 |
| タンパク質長 | Thr2-Thr116 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 40.65 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | LAPTM4B, Lysosomal-associated transmembrane protein 4B, Lysosome-associated transmembrane protein 4-beta |
| 背景 | Lysosomal-associated transmembrane protein 4B is a ~35 kDa protein. Required for optimal lysosomal function. Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation. Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation. Plays a role as negative regulator of TGFB1 production in regulatory T cells. 1. Vergarajauregui, S. et al. (2011) Journal of cell science 124, 459-68. PMID: 21224396 2. Tan, X. et al. (2015) The EMBO journal 34, 475-90. PMID: 25588945 3. Milkereit, R. et al. (2015) Nature communications 6, 7250. PMID: 25998567 4. Huygens, C. et al. (2015) The Journal of biological chemistry 290, 20105-16. PMID: 26126825 5. Blom, T. et al. (2015) Nature chemical biology 11, 799-806. PMID: 26280656 |
| 注意事項 | For research use only |
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
