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| カタログ番号 | HD734012 |
|---|---|
| 説明 |
Recombinant Human LATS2 Protein, N-His (HD734012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9NRM7 |
| タンパク質長 | Lys665-Ala1036 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | EC:2.7.11.1, KPM, Kinase phosphorylated during mitosis protein, LATS2, Large tumor suppressor homolog 2, Serine/threonine-protein kinase LATS2, Serine/threonine-protein kinase kpm, Warts-like kinase |
| 背景 | Serine/threonine-protein kinase LATS2 is a ~120 kDa protein. Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Also phosphorylates YAP1 in response to cell contact inhibition-driven WWP1 ubiquitination of AMOTL2, which results in LATS2 activation. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. 1. Hao, Y. et al. (2008) The Journal of biological chemistry 283, 5496-509. PMID: 18158288 2. Meng, Z. et al. (2015) Nature communications 6, 8357. PMID: 26437443 3. Kim, M. et al. (2016) EMBO reports 17, 64-78. PMID: 26598551 4. Hwang, D. et al. (2021) Life science alliance 4. PMID: 34404733 8. Zhang, K. et al. (2012) The EMBO journal 31, 29-43. PMID: 21952048 9. Nie, L. et al. (2024) Molecular cell 84, 3336-3353.e7. PMID: 39173637 |
| 注意事項 | For research use only |
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