Human LBR Recombinant Protein (N-GST & C-His) (HC667012)

Name: Human LBR Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HC667012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HC667012
Description	Recombinant Human LBR Protein, N-GST & C-His (HC667012) expressed in E. coli, spanning Ala7-Arg61. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q14739
Longueur de protéine	Ala7-Arg61
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	34.50 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	LMN2R, LBR, C14SR, Integral nuclear envelope inner membrane protein, Sterol C14-reductase, 3-beta-hydroxysterol Delta (14)-reductase, Delta(14)-sterol reductase LBR, Lamin-B receptor, Delta-14-SR, C-14 sterol reductase
Contexte	Delta(14)-sterol reductase LBR is a ~70 kDa protein. Catalyzes the reduction of the C14-unsaturated bond of lanosterol, as part of the metabolic pathway leading to cholesterol biosynthesis. Plays a critical role in myeloid cell cholesterol biosynthesis which is essential to both myeloid cell growth and functional maturation. Mediates the activation of NADPH oxidases, perhaps by maintaining critical levels of cholesterol required for membrane lipid raft formation during neutrophil differentiation. Anchors the lamina and the heterochromatin to the inner nuclear membrane. 1. Waterham, HR. et al. (2003) American journal of human genetics 72, 1013-7. PMID: 12618959 2. Bennati, AM. et al. (2006) Biochimica et biophysica acta 1761, 677-85. PMID: 16784888 3. Clayton, P. et al. (2010) Nucleus (Austin, Tex.) 1, 354-66. PMID: 21327084 4. Tsai, PL. et al. (2016) eLife 5. PMID: 27336722 5. Silve, S. et al. (1998) Biochimica et biophysica acta 1392, 233-44. PMID: 9630650 6. Duband-Goulet, I. et al. (2000) Biochemistry 39, 6483-8. PMID: 10828963
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human LBR Protein, N-GST & C-His

Reférences

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Human LBR Recombinant Protein (N-GST & C-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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