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| カタログ番号 | HW360012 |
|---|---|
| 説明 |
Recombinant Human LEP Protein, N-His (HW360012) expressed in E. coli, spanning Val22-Cys167. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P41159 |
| タンパク質長 | Val22-Cys167 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 18.32 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | OBS, OB, Obese protein, LEP, Leptin, Obesity factor |
| 背景 | Leptin is a ~18 kDa protein. Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways. In the hypothalamus, acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic for endothelial cell and affects innate and adaptive immunity. In the arcuate nucleus of the hypothalamus, activates by depolarization POMC neurons inducing FOS and SOCS3 expression to release anorexigenic peptides and inhibits by hyperpolarization NPY neurons inducing SOCS3 with a consequent reduction on release of orexigenic peptides. 1. Otero, M. et al. (2005) Arthritis research & therapy 7, R581-91. PMID: 15899045 2. Saxena, NK. et al. (2007) The Journal of biological chemistry 282, 13316-25. PMID: 17344214 3. Vuolteenaho, K. et al. (2009) Mediators of inflammation 2009, 345838. PMID: 19688109 4. Park, HY. et al. (2001) Experimental & molecular medicine 33, 95-102. PMID: 11460888 5. El-Zein, O. et al. (2013) PloS one 8, e83360. PMID: 24340098 6. Cassano, S. et al. (2014) Metabolism: clinical and experimental 63, 1272-9. PMID: 25060689 7. Chehab, FF. et al. (1996) Nature genetics 12, 318-20. PMID: 8589726 8. Jiang, H. et al. (2008) Biochemical and biophysical research communications 368, 1-5. PMID: 18242580 9. Zhao, Y. et al. (2003) Biochemical and biophysical research communications 300, 247-52. PMID: 12504075 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human LEP Protein, N-His
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