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| カタログ番号 | HW824012 |
|---|---|
| 説明 |
Recombinant Human LIG4 Protein, N-His (HW824012) expressed in E. coli, spanning Met1-Lys609. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P49917 |
| タンパク質長 | Met1-Lys609 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 71.50 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4, LIG4, DNA ligase 4 |
| 背景 | DNA ligase 4 (LIG4) is a ~103 kDa protein. DNA ligase involved in DNA non-homologous end joining (NHEJ); required for double-strand break (DSB) repair and V(D)J recombination. Catalyzes the NHEJ ligation step of the broken DNA during DSB repair by resealing the DNA breaks after the gap filling is completed. Joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. LIG4 is mechanistically flexible: it can ligate nicks as well as compatible DNA overhangs alone, while in the presence of XRCC4, it can ligate ends with 2-nucleotides (nt) microhomology and 1-nt gaps. Forms a subcomplex with XRCC4; the LIG4-XRCC4 subcomplex is responsible for the NHEJ ligation step and XRCC4 enhances the joining activity of LIG4. 1. Lee, JW. et al. (2003) Cancer research 63, 22-4. PMID: 12517771 2. Gu, J. et al. (2007) The EMBO journal 26, 1010-23. PMID: 17290226 3. Ochi, T. et al. (2013) Structure (London, England : 1993) 21, 672-9. PMID: 23523427 4. Kaminski, AM. et al. (2018) Nature communications 9, 2642. PMID: 29980672 5. Maddi, ER. et al. (2021) Protein engineering, design & selection : PEDS 34. PMID: 33586762 6. Robins, P. et al. (1996) The Journal of biological chemistry 271, 24257-61. PMID: 8798671 7. Grawunder, U. et al. (1997) Nature 388, 492-5. PMID: 9242410 8. Grawunder, U. et al. (1998) Molecular cell 2, 477-84. PMID: 9809069 9. Chen, L. et al. (2000) The Journal of biological chemistry 275, 26196-205. PMID: 10854421 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human LIG4 Protein, N-His
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