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| カタログ番号 | HP172012 |
|---|---|
| 説明 |
Recombinant Human LIN28A Protein, N-His-SUMO & C-Strep (HP172012) expressed in E. coli, spanning Gln36-Gly111. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9H9Z2 |
| タンパク質長 | Gln36-Gly111 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 21.99 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | CSDD1, Zinc finger CCHC domain-containing protein 1, LIN28, LIN28A, Lin-28A, Protein lin-28 homolog A, ZCCHC1 |
| 背景 | Protein lin-28 homolog A (LIN28A) is a ~22 kDa protein. RNA-binding protein that inhibits processing of pre-let-7 miRNAs and regulates translation of mRNAs that control developmental timing, pluripotency and metabolism. Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets (Probable). 'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. 1. Jin, J. et al. (2011) Nucleic acids research 39, 3724-34. PMID: 21247876 2. Heo, I. et al. (2008) Molecular cell 32, 276-84. PMID: 18951094 3. Heo, I. et al. (2009) Cell 138, 696-708. PMID: 19703396 4. Piskounova, E. et al. (2011) Cell 147, 1066-79. PMID: 22118463 5. Thornton, JE. et al. (2012) RNA (New York, N.Y.) 18, 1875-85. PMID: 22898984 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human LIN28A Protein, N-His-SUMO & C-Strep
+86-027-65523339
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