Human LIPC Recombinant Protein (N-His) (HY234012)

Name: Human LIPC Recombinant Protein (N-His)
Brand: abinScience
SKU: HY234012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HY234012
Description	Recombinant Human LIPC Protein, N-His (HY234012) expressed in E. coli, spanning Glu47-Pro478. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	P11150
Longueur de protéine	Glu47-Pro478
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	50.6 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Phospholipase A1, Lipase member C, LIPC, Hepatic lipase, Hepatic triacylglycerol lipase, Lysophospholipase, HTGL, HL
Contexte	Hepatic triacylglycerol lipase (LIPC) is a ~55 kDa protein. Catalyzes the hydrolysis of triglycerides and phospholipids present in circulating plasma lipoproteins, including chylomicrons, intermediate density lipoproteins (IDL), low density lipoproteins (LDL) of large size and high density lipoproteins (HDL), releasing free fatty acids (FFA) and smaller lipoprotein particles. Also exhibits lysophospholipase activity. Can hydrolyze both neutral lipid and phospholipid substrates but shows a greater binding affinity for neutral lipid substrates than phospholipid substrates. In native LDL, preferentially hydrolyzes the phosphatidylcholine species containing polyunsaturated fatty acids at sn-2 position. 1. McCoy, MG. et al. (2002) Journal of lipid research 43, 921-9. PMID: 12032167 2. Yang, P. et al. (2015) Biochimica et biophysica acta 1851, 1327-36. PMID: 26193433 3. Dugi, KA. et al. (1995) The Journal of biological chemistry 270, 25396-401. PMID: 7592706 4. Hill, JS. et al. (1996) The Journal of biological chemistry 271, 22931-6. PMID: 8798474
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human LIPC Protein, N-His

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Human LIPC Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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