

| Numéro de catalogue | HC968012 |
|---|---|
| Description |
Recombinant Human LTN1 Protein, N-His (HC968012) expressed in E. coli, spanning Glu1587-Phe1766. Purity: >90% by SDS-PAGE.
Highlights
|
| Système d'expression | E. coli |
| Numéro d'accès | O94822 |
| Longueur de protéine | Glu1587-Phe1766 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Espèces | Homo sapiens (Human) |
| Nature | Recombinant |
| Niveau d'endotoxines | Please contact with the lab for this information. |
| Pureté | >90% as determined by SDS-PAGE. |
| Masse moléculaire prédite | 22.96 kDa |
| Forme | Lyophilized |
| Tampon de stockage | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Expédition | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stabilité et stockage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Noms alternatifs | C21orf10, C21orf98, E3 ubiquitin-protein ligase listerin, EC:2.3.2.27, KIAA0714, LTN1, RING finger protein 160, RING-type E3 ubiquitin transferase listerin, RNF160, ZNF294, Zinc finger protein 294 |
| Contexte | E3 ubiquitin-protein ligase listerin is a ~200 kDa protein. E3 ubiquitin-protein ligase component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. Within the RQC complex, LTN1 is recruited to stalled 60S ribosomal subunits by NEMF and mediates ubiquitination of stalled nascent chains. Ubiquitination leads to VCP/p97 recruitment for extraction and degradation of the incomplete translation product. 1. Shao, S. et al. (2013) Molecular cell 50, 637-48. PMID: 23685075 2. Shao, S. et al. (2014) Molecular cell 55, 880-890. PMID: 25132172 3. Shao, S. et al. (2015) Molecular cell 57, 433-44. PMID: 25578875 4. Matsuo, Y. et al. (2017) Nature communications 8, 159. PMID: 28757607 |
| Note | For research use only |
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