Human MAP1LC3A Recombinant Protein (N-His) (HV978012)

Name: Human MAP1LC3A Recombinant Protein (N-His)
Brand: abinScience
SKU: HV978012
Price: 211 USD
Availability: InStock

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1mg

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Vista general

Número de catálogo	HV978012
Descripción	Recombinant Human MAP1LC3A Protein, N-His (HV978012) expressed in E. coli, spanning Met1-Gly120. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q9H492
Longitud de proteína	Met1-Gly120
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	16.42 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, Microtubule-associated protein 1 light chain 3 alpha, MAP1A/MAP1B LC3 A, MAP1LC3A, Microtubule-associated proteins 1A/1B light chain 3A, MAP1A/MAP1B light chain 3 A
Fondo	Microtubule-associated protein 1 light chain 3 alpha (MAP1LC3A) is a ~14 kDa protein. Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover. 1. Cherra, SJ. et al. (2010) The Journal of cell biology 190, 533-9. PMID: 20713600 2. Suzuki, H. et al. (2014) Structure (London, England : 1993) 22, 47-58. PMID: 24290141 3. An, H. et al. (2019) Molecular cell 74, 891-908.e10. PMID: 31006537 4. Chino, H. et al. (2019) Molecular cell 74, 909-921.e6. PMID: 31006538
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human MAP1LC3A Protein, N-His

Referencias

Datasheet

Human MAP1LC3A Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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