Human MCM8 Recombinant Protein (N-His) (HP407012)

Name: Human MCM8 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP407012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HP407012
Description	Recombinant Human MCM8 Protein, N-His (HP407012) expressed in E. coli, spanning Ser90-Lys366. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q9UJA3
Longueur de protéine	Ser90-Lys366
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	33.26 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	MCM8, C20orf154, Minichromosome maintenance 8, DNA helicase MCM8
Contexte	DNA helicase MCM8 is a ~93 kDa protein. Component of the MCM8-MCM9 complex, which is involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR). The MCM8-MCM9 complex is a 3'-5' DNA helicase and single-stranded (ss)DNA-stimulated ATPase which binds ssDNA in the presence of nucleoside triphosphates. Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity. Indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs, probably by regulating the localization of the MNR complex. The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression. 1. Park, J. et al. (2013) Molecular and cellular biology 33, 1632-44. PMID: 23401855 2. McKinzey, DR. et al. (2023) Nucleic acids research 51, 7330-7341. PMID: 37309874 3. Lee, KY. et al. (2015) Nature communications 6, 7744. PMID: 26215093 4. Volkening, M. et al. (2005) Molecular and cellular biology 25, 1560-8. PMID: 15684404 5. Klucnika, A. et al. (2023) The Journal of cell biology 222. PMID: 36355348
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human MCM8 Protein, N-His

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Human MCM8 Recombinant Protein (N-His).pdf

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