ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды
Homo sapiens (Human)
Характер
Recombinant
Уровень эндотоксинов
Please contact with the lab for this information.
Чистота
>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса
25.94 kDa
Форма
Lyophilized
Буфер хранения
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия
Activator-recruited cofactor 205 kDa component, DRIP230, TRIP-2, TR-interacting protein 2, CRSP200, Vitamin D receptor-interacting protein complex component DRIP205, ARC205, MED1, Mediator complex subunit 1, PPAR-binding protein, TRAP220, TRIP2, Thyroid hormone receptor-associated protein complex 220 kDa component, PPARGBP, Mediator of RNA polymerase II transcription subunit 1, DRIP205, Trap220, RB18A, CRSP1, Peroxisome proliferator-activated receptor-binding protein, PPARBP, Thyroid receptor-interacting protein 2, p53 regulatory protein RB18A, PBP
Фон
Mediator of RNA polymerase II transcription subunit 1 (MED1) is a ~168 kDa protein. Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells.
1. Zhang, J. et al. (1999) Molecular endocrinology (Baltimore, Md.) 13, 1130-40. PMID: 10406464
2. Kang, YK. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 2642-7. PMID: 11867769
3. Ge, K. et al. (2002) Nature 417, 563-7. PMID: 12037571
4. Wang, Q. et al. (2002) The Journal of biological chemistry 277, 42852-8. PMID: 12218053
5. Coulthard, VH. et al. (2003) The Journal of biological chemistry 278, 10942-51. PMID: 12556447
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