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| カタログ番号 | HP166012 |
|---|---|
| 説明 |
Recombinant Human METTL1 Protein, N-His (HP166012) expressed in E. coli, spanning Thr34-His276. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9UBP6 |
| タンパク質長 | Thr34-His276 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 30.06 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | miRNA (guanine-N(7)-)-methyltransferase, Methyltransferase-like protein 1, tRNA (guanine-N(7)-)-methyltransferase, mRNA (guanine-N(7)-)-methyltransferase, C12orf1, tRNA (guanine(46)-N(7))-methyltransferase, METTL1, tRNA(m7G46)-methyltransferase |
| 背景 | tRNA (guanine-N(7)-)-methyltransferase (METTL1) is a ~31 kDa protein. Catalytic component of METTL1-WDR4 methyltransferase complex that mediates the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs). Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in a large subset of tRNAs that contain the 5'-RAGGU-3' motif within the variable loop. M7G46 interacts with C13-G22 in the D-loop to stabilize tRNA tertiary structure and protect tRNAs from decay. Also acts as a methyltransferase for a subset of internal N(7)-methylguanine in mRNAs. Internal N(7)-methylguanine methylation of mRNAs in response to stress promotes their relocalization to stress granules, thereby suppressing their translation. 1. Alexandrov, A. et al. (2002) RNA (New York, N.Y.) 8, 1253-66. PMID: 12403464 2. Pandolfini, L. et al. (2019) Molecular cell 74, 1278-1290.e9. PMID: 31031083 3. Zhang, LS. et al. (2019) Molecular cell 74, 1304-1316.e8. PMID: 31031084 4. Ruiz-Arroyo, VM. et al. (2023) Nature 613, 383-390. PMID: 36599982 5. Li, J. et al. (2023) Nature 613, 391-397. PMID: 36599985 6. Jin, X. et al. (2023) Cell discovery 9, 65. PMID: 37369656 7. Zhao, Z. et al. (2023) Cell 186, 3208-3226.e27. PMID: 37379838 8. Dai, Z. et al. (2021) Molecular cell 81, 3339-3355.e8. PMID: 34352206 9. Orellana, EA. et al. (2021) Molecular cell 81, 3323-3338.e14. PMID: 34352207 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human METTL1 Protein, N-His
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中国武漢市深敦寺路666号C棟、武漢、430206
