Human METTL14 Recombinant Protein (N-His) (HC797012)

Name: Human METTL14 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC797012
Price: 211 USD
Availability: InStock

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Número de catálogo	HC797012
Descripción	Recombinant Human METTL14 Protein, N-His (HC797012) expressed in E. coli, spanning Pro147-Ser399. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q9HCE5
Longitud de proteína	Pro147-Ser399
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	31.51 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	KIAA1627, METTL14, Methyltransferase-like protein 14, N(6)-adenosine-methyltransferase non-catalytic subunit METTL14, hMETTL14
Fondo	N(6)-adenosine-methyltransferase non-catalytic subunit METTL14 is a ~52 kDa protein. The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis. In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing. M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation. In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization. 1. Liu, J. et al. (2014) Nature chemical biology 10, 93-5. PMID: 24316715 2. Ping, XL. et al. (2014) Cell research 24, 177-89. PMID: 24407421 3. Liu, N. et al. (2015) Nature 518, 560-4. PMID: 25719671 4. Wang, X. et al. (2016) Nature 534, 575-8. PMID: 27281194 5. Wang, P. et al. (2016) Molecular cell 63, 306-317. PMID: 27373337 6. Schöller, E. et al. (2018) RNA (New York, N.Y.) 24, 499-512. PMID: 29348140 7. Śledź, P. et al. (2016) eLife 5. PMID: 27627798
Nota	For research use only

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Datasheet

Human METTL14 Recombinant Protein (N-His).pdf

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