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| カタログ番号 | HC797012 |
|---|---|
| 説明 |
Recombinant Human METTL14 Protein, N-His (HC797012) expressed in E. coli, spanning Pro147-Ser399. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9HCE5 |
| タンパク質長 | Pro147-Ser399 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 31.51 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | KIAA1627, METTL14, Methyltransferase-like protein 14, N(6)-adenosine-methyltransferase non-catalytic subunit METTL14, hMETTL14 |
| 背景 | N(6)-adenosine-methyltransferase non-catalytic subunit METTL14 is a ~52 kDa protein. The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis. In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing. M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation. In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization. 1. Liu, J. et al. (2014) Nature chemical biology 10, 93-5. PMID: 24316715 2. Ping, XL. et al. (2014) Cell research 24, 177-89. PMID: 24407421 3. Liu, N. et al. (2015) Nature 518, 560-4. PMID: 25719671 4. Wang, X. et al. (2016) Nature 534, 575-8. PMID: 27281194 5. Wang, P. et al. (2016) Molecular cell 63, 306-317. PMID: 27373337 6. Schöller, E. et al. (2018) RNA (New York, N.Y.) 24, 499-512. PMID: 29348140 7. Śledź, P. et al. (2016) eLife 5. PMID: 27627798 |
| 注意事項 | For research use only |
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