Human METTL3 Recombinant Protein (C-His) (HV489011)

Name: Human METTL3 Recombinant Protein (C-His)
Brand: abinScience
SKU: HV489011
Price: 298 USD
Availability: InStock

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Numéro de catalogue	HV489011
Description	Recombinant Human METTL3 Protein, C-His (HV489011) expressed in Insect Cells, spanning Met1-Leu580. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	Insect cells
Numéro d'accès	Q86U44
Longueur de protéine	Met1-Leu580
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	65.44 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	EC:2.1.1.348, METTL3, MT-A70, MTA70, Methyltransferase-like protein 3, N(6)-adenosine-methyltransferase 70 kDa subunit, N(6)-adenosine-methyltransferase catalytic subunit METTL3, hMETTL3
Contexte	N(6)-adenosine-methyltransferase catalytic subunit METTL3 is a ~64 kDa protein. The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some RNAs and regulates various processes such as the circadian clock, differentiation of embryonic and hematopoietic stem cells, cortical neurogenesis, response to DNA damage, differentiation of T-cells and primary miRNA processing. In the heterodimer formed with METTL14, METTL3 constitutes the catalytic core. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability, processing, translation efficiency and editing. M6A acts as a key regulator of mRNA stability: methylation is completed upon the release of mRNA into the nucleoplasm and promotes mRNA destabilization and degradation. In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization, promoting differentiation of ESCs. 1. Dominissini, D. et al. (2012) Nature 485, 201-6. PMID: 22575960 2. Wang, X. et al. (2014) Nature 505, 117-20. PMID: 24284625 3. Liu, N. et al. (2015) Nature 518, 560-4. PMID: 25719671 4. Alarcón, CR. et al. (2015) Nature 519, 482-5. PMID: 25799998 5. Alarcón, CR. et al. (2015) Cell 162, 1299-308. PMID: 26321680 6. Meyer, KD. et al. (2015) Cell 163, 999-1010. PMID: 26593424 7. Wang, X. et al. (2016) Nature 534, 575-8. PMID: 27281194 8. Wang, P. et al. (2016) Molecular cell 63, 306-317. PMID: 27373337 9. Śledź, P. et al. (2016) eLife 5. PMID: 27627798 10. Xiang, Y. et al. (2017) Nature 543, 573-576. PMID: 28297716
Note	For research use only