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| カタログ番号 | HV489011 |
|---|---|
| 説明 |
Recombinant Human METTL3 Protein, C-His (HV489011) expressed in Insect Cells, spanning Met1-Leu580. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | Insect cells |
| アクセッション番号 | Q86U44 |
| タンパク質長 | Met1-Leu580 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 65.44 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | EC:2.1.1.348, METTL3, MT-A70, MTA70, Methyltransferase-like protein 3, N(6)-adenosine-methyltransferase 70 kDa subunit, N(6)-adenosine-methyltransferase catalytic subunit METTL3, hMETTL3 |
| 背景 | N(6)-adenosine-methyltransferase catalytic subunit METTL3 is a ~64 kDa protein. The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some RNAs and regulates various processes such as the circadian clock, differentiation of embryonic and hematopoietic stem cells, cortical neurogenesis, response to DNA damage, differentiation of T-cells and primary miRNA processing. In the heterodimer formed with METTL14, METTL3 constitutes the catalytic core. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability, processing, translation efficiency and editing. M6A acts as a key regulator of mRNA stability: methylation is completed upon the release of mRNA into the nucleoplasm and promotes mRNA destabilization and degradation. In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization, promoting differentiation of ESCs. 1. Dominissini, D. et al. (2012) Nature 485, 201-6. PMID: 22575960 2. Wang, X. et al. (2014) Nature 505, 117-20. PMID: 24284625 3. Liu, N. et al. (2015) Nature 518, 560-4. PMID: 25719671 4. Alarcón, CR. et al. (2015) Nature 519, 482-5. PMID: 25799998 5. Alarcón, CR. et al. (2015) Cell 162, 1299-308. PMID: 26321680 6. Meyer, KD. et al. (2015) Cell 163, 999-1010. PMID: 26593424 7. Wang, X. et al. (2016) Nature 534, 575-8. PMID: 27281194 8. Wang, P. et al. (2016) Molecular cell 63, 306-317. PMID: 27373337 9. Śledź, P. et al. (2016) eLife 5. PMID: 27627798 10. Xiang, Y. et al. (2017) Nature 543, 573-576. PMID: 28297716 |
| 注意事項 | For research use only |
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