Human METTL3 Recombinant Protein (C-His) (HV489011)

Name: Human METTL3 Recombinant Protein (C-His)
Brand: abinScience
SKU: HV489011
Price: 298 USD
Availability: InStock

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100ug
1mg

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Каталожный номер	HV489011
Описание	Recombinant Human METTL3 Protein, C-His (HV489011) expressed in Insect Cells, spanning Met1-Leu580. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	Insect cells
Номер доступа	Q86U44
Длина белка	Met1-Leu580
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	65.44 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	EC:2.1.1.348, METTL3, MT-A70, MTA70, Methyltransferase-like protein 3, N(6)-adenosine-methyltransferase 70 kDa subunit, N(6)-adenosine-methyltransferase catalytic subunit METTL3, hMETTL3
Фон	N(6)-adenosine-methyltransferase catalytic subunit METTL3 is a ~64 kDa protein. The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some RNAs and regulates various processes such as the circadian clock, differentiation of embryonic and hematopoietic stem cells, cortical neurogenesis, response to DNA damage, differentiation of T-cells and primary miRNA processing. In the heterodimer formed with METTL14, METTL3 constitutes the catalytic core. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability, processing, translation efficiency and editing. M6A acts as a key regulator of mRNA stability: methylation is completed upon the release of mRNA into the nucleoplasm and promotes mRNA destabilization and degradation. In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization, promoting differentiation of ESCs. 1. Dominissini, D. et al. (2012) Nature 485, 201-6. PMID: 22575960 2. Wang, X. et al. (2014) Nature 505, 117-20. PMID: 24284625 3. Liu, N. et al. (2015) Nature 518, 560-4. PMID: 25719671 4. Alarcón, CR. et al. (2015) Nature 519, 482-5. PMID: 25799998 5. Alarcón, CR. et al. (2015) Cell 162, 1299-308. PMID: 26321680 6. Meyer, KD. et al. (2015) Cell 163, 999-1010. PMID: 26593424 7. Wang, X. et al. (2016) Nature 534, 575-8. PMID: 27281194 8. Wang, P. et al. (2016) Molecular cell 63, 306-317. PMID: 27373337 9. Śledź, P. et al. (2016) eLife 5. PMID: 27627798 10. Xiang, Y. et al. (2017) Nature 543, 573-576. PMID: 28297716
Примечание	For research use only