Human MFN2 Recombinant Protein (N-His) (HF769012)

Name: Human MFN2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HF769012
Price: 211 USD
Availability: InStock

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Numéro de catalogue	HF769012
Description	Recombinant Human MFN2 Protein, N-His (HF769012) expressed in E. coli, spanning His20-Arg400. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	O95140
Longueur de protéine	His20-Arg400
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	45.36 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Transmembrane GTPase MFN2, Mitofusin-2, KIAA0214, CPRP1, MFN2
Contexte	Mitofusin-2 (MFN2) is a ~86 kDa protein. Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks. Membrane clustering requires GTPase activity and may involve a major rearrangement of the coiled coil domains (Probable). Plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. 1. Santel, A. et al. (2001) Journal of cell science 114, 867-74. PMID: 11181170 2. Rojo, M. et al. (2002) Journal of cell science 115, 1663-74. PMID: 11950885 3. Casasnovas, C. et al. (2010) Journal of medical genetics 47, 249-56. PMID: 19889647 4. Senyilmaz, D. et al. (2015) Nature 525, 124-8. PMID: 26214738 5. Cao, YL. et al. (2017) Nature 542, 372-376. PMID: 28114303 6. Chen, Y. et al. (2013) Science (New York, N.Y.) 340, 471-5. PMID: 23620051
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human MFN2 Protein, N-His

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Human MFN2 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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