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Обзор
Каталожный номерHD501012
Описание
Recombinant Human MIEF1 Protein, N-GST & C-His (HD501012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
Система экспрессииE. coli
Номер доступаL0R8F8
Длина белкаAla2-Ile70
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыHomo sapiens (Human)
Характер Recombinant
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияAltMIEF1, AltMiD51, Alternative MIEF1 protein, MIEF1, MIEF1 microprotein, MIEF1-MP, Mitochondrial ribosome and complex I assembly factor AltMIEF1, alternative transcript upstream of MiD51
Фон

Mitochondrial ribosome and complex I assembly factor AltMIEF1 is a ~8 kDa protein. Assembly factor involved in the biogenesis of the mitochondrial-specific ribosomes (mitoribosomes). Specifically associates with intermediates of the mitochondrial ribosome large subunit (mt-LSU) and is required for proper ribosome assembly, possibly preventing premature association of the large and small ribosomal subunits. Thereby, indirectly regulates mitochondrial translation. It is also required for complete assembly of the mitochondrial respiratory chain complex I. May also function in DNM1L-mediated mitochondrial fission.

1. Brown, A. et al. (2017) Nature structural & molecular biology 24, 866-869. PMID: 28892042
2. Rathore, A. et al. (2018) Biochemistry 57, 5564-5575. PMID: 30215512
3. Dibley, MG. et al. (2020) Molecular & cellular proteomics : MCP 19, 65-77. PMID: 31666358
Примечание For research use only
Изображения
Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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