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| カタログ番号 | HV195012 |
|---|---|
| 説明 |
Recombinant Human MLST8 Protein, N-His (HV195012) expressed in E. coli, spanning Tyr68-Trp247. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9BVC4 |
| タンパク質長 | Tyr68-Trp247 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 22.3 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | GBL, TORC subunit LST8, Mammalian lethal with SEC13 protein 8, G protein beta subunit-like, Gable, Protein GbetaL, mLST8, LST8, MLST8, Target of rapamycin complex subunit LST8 |
| 背景 | Target of rapamycin complex subunit LST8 (MLST8) is a ~35 kDa protein. Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. In response to nutrients, mTORC1 is recruited to the lysosome membrane and promotes protein, lipid and nucleotide synthesis by phosphorylating several substrates, such as ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1). In the same time, it inhibits catabolic pathways by phosphorylating the autophagy initiation components ULK1 and ATG13, as well as transcription factor TFEB, a master regulators of lysosomal biogenesis and autophagy. The mTORC1 complex is inhibited in response to starvation and amino acid depletion. 1. Kim, DH. et al. (2003) Molecular cell 11, 895-904. PMID: 12718876 2. Sarbassov, DD. et al. (2004) Current biology : CB 14, 1296-302. PMID: 15268862 3. Jacinto, E. et al. (2004) Nature cell biology 6, 1122-8. PMID: 15467718 4. Coffman, K. et al. (2014) The Journal of biological chemistry 289, 4723-34. PMID: 24403073 5. Wang, B. et al. (2017) Nature 545, 365-369. PMID: 28489822 6. Yu, Z. et al. (2022) The Journal of biological chemistry 298, 102288. PMID: 35926713 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human MLST8 Protein, N-His
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中国武漢市深敦寺路666号C棟、武漢、430206
