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| カタログ番号 | HA308012 |
|---|---|
| 説明 |
Recombinant Human MR1 Protein, C-Avi (HA308012) expressed in E. coli, spanning Arg23-Pro292. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q95460 |
| タンパク質長 | Arg23-Pro292 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 33.64 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Class I histocompatibility antigen-like protein, MR1, MHC class I-related gene protein, Major histocompatibility complex class I-related gene protein |
| 背景 | Major histocompatibility complex class I-related protein 1 (MR1) is a ~39 kDa protein. Antigen-presenting molecule specialized in displaying microbial pyrimidine-based metabolites to alpha-beta T cell receptors (TCR) on innate-type mucosal-associated invariant T (MAIT) cells. In complex with B2M preferentially presents riboflavin-derived metabolites to semi-invariant TRAV1.2 TCRs on MAIT cells, guiding immune surveillance of the microbial metabolome at mucosal epithelial barriers. Signature pyrimidine-based microbial antigens are generated via non-enzymatic condensation of metabolite intermediates of the riboflavin pathway with by-products arising from other metabolic pathways such as glycolysis. Typical potent antigenic metabolites are 5-(2-oxoethylideneamino)-6-D-ribitylaminouracil (5-OE-RU) and 5-(2-oxopropylideneamino)-6-D-ribitylaminouracil (5-OP-RU), products of condensation of 5-amino-6-D-ribityaminouracil (5-A-RU) with glyoxal or methylglyoxal by-products, respectively. May present microbial antigens to various TRAV1-2-negative MAIT cell subsets, providing for unique recognition of diverse microbes, including pathogens that do not synthesize riboflavin. 1. Huang, S. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 8290-5. PMID: 19416870 2. Lion, J. et al. (2013) European journal of immunology 43, 1363-73. PMID: 23457030 3. Gold, MC. et al. (2013) Mucosal immunology 6, 35-44. PMID: 22692454 4. Kjer-Nielsen, L. et al. (2012) Nature 491, 717-23. PMID: 23051753 5. Reantragoon, R. et al. (2013) The Journal of experimental medicine 210, 2305-20. PMID: 24101382 6. Patel, O. et al. (2013) Nature communications 4, 2142. PMID: 23846752 7. Gherardin, NA. et al. (2016) Immunity 44, 32-45. PMID: 26795251 8. Le Bourhis, L. et al. (2010) Nature immunology 11, 701-8. PMID: 20581831 9. Corbett, AJ. et al. (2014) Nature 509, 361-5. PMID: 24695216 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human MR1 Protein, C-Avi
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