Human MRAS Recombinant Protein (N-His) (HB573012)

Name: Human MRAS Recombinant Protein (N-His)
Brand: abinScience
SKU: HB573012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Каталожный номер	HB573012
Описание	Recombinant Human MRAS Protein, N-His (HB573012) expressed in E. coli, spanning Met1-Gln204. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	O14807
Длина белка	Met1-Gln204
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	25.58 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	Ras-related protein M-Ras; 3.6.5.2; Ras-related protein R-Ras3; MRAS; RRAS3
Фон	Ras-related protein M-Ras is a ~23 kDa protein. Signal transducer in the Ras-MAPK signaling pathway that regulates cell proliferation and survival. Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates the MAPK pathway activation. The formation of the SMP complex only occurs when MRAS is GTP-bound. MRAS has low intrinsic GTPase activity and may require additional factors for activation. The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities. 1. Rodriguez-Viciana, P. et al. (2006) Molecular cell 22, 217-30. PMID: 16630891 2. Higgins, EM. et al. (2017) JCI insight 2, e91225. PMID: 28289718 3. Liau, NPD. et al. (2022) Nature 609, 400-407. PMID: 35768504 4. Hauseman, ZJ. et al. (2022) Nature 609, 416-423. PMID: 35830882 5. Kwon, JJ. et al. (2022) Nature 609, 408-415. PMID: 35831509 6. Bonsor, DA. et al. (2022) Nature structural & molecular biology 29, 966-977. PMID: 36175670 7. Bernal Astrain, G. et al. (2025) Nature communications 16, 647. PMID: 39809765
Примечание	For research use only

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human MRAS Protein, N-His

Ссылки

Даташит

Human MRAS Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ (Stock Conc.)

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