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Human MRPL12 Recombinant Protein (N-GST & C-His) (HC170012)

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Vista general
Número de catálogoHC170012
Descripción
Recombinant Human MRPL12 Protein, N-GST & C-His (HC170012) expressed in E. coli, spanning His129-Glu198. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresiónE. coli
Número de accesoP52815
Longitud de proteínaHis129-Glu198
AplicacionesELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspeciesHomo sapiens (Human)
Naturaleza Recombinant
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >90% as determined by SDS-PAGE.
Peso molecular predicho 35.85 kDa
Forma Lyophilized
Buffer de almacenamiento Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Reconstitución Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Envío In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos39S ribosomal protein L12, mitochondrial, 5c5-2, L12mt, Large ribosomal subunit protein bL12m, Large ribosomal subunit protein bL12m, long mature form, Large ribosomal subunit protein bL12m, short mature form, MRP-L12, MRPL12, MRPL7, RPML12
Fondo

Large ribosomal subunit protein bL12m is a ~21 kDa protein. As a component of the mitochondrial large ribosomal subunit, plays a role in mitochondrial translation. When present in mitochondria as a free protein not associated with the ribosome, associates with mitochondrial RNA polymerase POLRMT to activate transcription. Required for POLRMT stability.

1. Serre, V. et al. (2013) Biochimica et biophysica acta 1832, 1304-12. PMID: 23603806
2. Surovtseva, YV. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 17921-6. PMID: 22003127
3. Nouws, J. et al. (2016) The Journal of biological chemistry 291, 989-97. PMID: 26586915
Nota For research use only
Imágenes
Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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