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Human MRPL12 Recombinant Protein (N-GST & C-His) (HC170012)

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Aperçu
Numéro de catalogueHC170012
Description
Recombinant Human MRPL12 Protein, N-GST & C-His (HC170012) expressed in E. coli, spanning His129-Glu198. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expressionE. coli
Numéro d'accèsP52815
Longueur de protéineHis129-Glu198
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspècesHomo sapiens (Human)
Nature Recombinant
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Masse moléculaire prédite 35.85 kDa
Forme Lyophilized
Tampon de stockage Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Expédition In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs39S ribosomal protein L12, mitochondrial, 5c5-2, L12mt, Large ribosomal subunit protein bL12m, Large ribosomal subunit protein bL12m, long mature form, Large ribosomal subunit protein bL12m, short mature form, MRP-L12, MRPL12, MRPL7, RPML12
Contexte

Large ribosomal subunit protein bL12m is a ~21 kDa protein. As a component of the mitochondrial large ribosomal subunit, plays a role in mitochondrial translation. When present in mitochondria as a free protein not associated with the ribosome, associates with mitochondrial RNA polymerase POLRMT to activate transcription. Required for POLRMT stability.

1. Serre, V. et al. (2013) Biochimica et biophysica acta 1832, 1304-12. PMID: 23603806
2. Surovtseva, YV. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 17921-6. PMID: 22003127
3. Nouws, J. et al. (2016) The Journal of biological chemistry 291, 989-97. PMID: 26586915
Note For research use only
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Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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