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| カタログ番号 | HW733022 |
|---|---|
| 説明 |
Recombinant Human MTOR Protein, N-His (HW733022) expressed in E. coli, spanning Ser27-Gln291. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P42345 |
| タンパク質長 | Ser27-Gln291 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 32.54 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Serine/threonine-protein kinase mTOR, 2.7.11.1, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, mTOR, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, MTOR, FRAP, FRAP1, FRAP2, RAFT1, RAPT1 |
| 背景 | Serine/threonine-protein kinase mTOR is a ~288 kDa protein. Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). In response to nutrients, growth factors or amino acids, mTORC1 is recruited to the lysosome membrane and promotes protein, lipid and nucleotide synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E). 1. Park, IH. et al. (2002) The Journal of biological chemistry 277, 31423-9. PMID: 12087098 2. Kim, DH. et al. (2002) Cell 110, 163-75. PMID: 12150925 3. Hara, K. et al. (2002) Cell 110, 177-89. PMID: 12150926 4. Choi, JH. et al. (2002) EMBO reports 3, 988-94. PMID: 12231510 5. Kim, DH. et al. (2003) Molecular cell 11, 895-904. PMID: 12718876 6. Inoki, K. et al. (2003) Cell 115, 577-90. PMID: 14651849 7. Sarbassov, DD. et al. (2004) Current biology : CB 14, 1296-302. PMID: 15268862 8. Jacinto, E. et al. (2004) Nature cell biology 6, 1122-8. PMID: 15467718 9. Brugarolas, J. et al. (2004) Genes & development 18, 2893-904. PMID: 15545625 10. Sarbassov, DD. et al. (2005) Science (New York, N.Y.) 307, 1098-101. PMID: 15718470 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human MTOR Protein, N-His
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中国武漢市深敦寺路666号C棟、武漢、430206
