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| カタログ番号 | HF552012 |
|---|---|
| 説明 |
Recombinant Human NBN Protein, N-His (HF552012) expressed in E. coli, spanning Met1-Asp121. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | O60934 |
| タンパク質長 | Met1-Asp121 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 15.42 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Nibrin, P95, NBS1, Cell cycle regulatory protein p95, NBS, Nijmegen breakage syndrome protein 1, NBN |
| 背景 | Nibrin (NBN) is a ~84 kDa protein. Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. The MRN complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11, to initiate end resection, which is required for single-strand invasion and recombination. Within the MRN complex, NBN acts as a protein-protein adapter, which specifically recognizes and binds phosphorylated proteins, promoting their recruitment to DNA damage sites. 1. Zhu, XD. et al. (2000) Nature genetics 25, 347-52. PMID: 10888888 2. Stiff, T. et al. (2005) The EMBO journal 24, 199-208. PMID: 15616588 3. Melander, F. et al. (2008) The Journal of cell biology 181, 213-26. PMID: 18411307 4. Chapman, JR. et al. (2008) EMBO reports 9, 795-801. PMID: 18583988 5. Wu, L. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 11200-5. PMID: 18678890 6. Yuan, J. et al. (2009) The Journal of biological chemistry 284, 31746-52. PMID: 19759395 7. Lu, CS. et al. (2012) The Journal of biological chemistry 287, 43984-94. PMID: 23115235 8. Rai, R. et al. (2017) Molecular cell 65, 801-817.e4. PMID: 28216226 9. Myler, LR. et al. (2017) Molecular cell 67, 891-898.e4. PMID: 28867292 10. Trujillo, KM. et al. (1998) The Journal of biological chemistry 273, 21447-50. PMID: 9705271 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human NBN Protein, N-His
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