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| カタログ番号 | HC741012 |
|---|---|
| 説明 |
Recombinant Human NEMF Protein, N-His (HC741012) expressed in E. coli, spanning Asp959-Lys1076. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | O60524 |
| タンパク質長 | Asp959-Lys1076 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 15.56 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Antigen NY-CO-1, NEMF, Nuclear export mediator factor, Ribosome quality control complex subunit NEMF, SDCCAG1, Serologically defined colon cancer antigen 1 |
| 背景 | Ribosome quality control complex subunit NEMF is a ~122 kDa protein. Key component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates the extraction of incompletely synthesized nascent chains from stalled ribosomes as well as their ubiquitin-mediated proteasomal degradation. Thereby, frees 60S subunit ribosomes from the stalled translation complex and prevents the accumulation of nascent polypeptide chains that are potentially toxic for the cell. Within the RQC complex, NEMF specifically binds stalled 60S ribosomal subunits by recognizing an exposed, nascent chain-conjugated tRNA moiety and promotes the recruitment of LTN1 to stalled 60S subunits. Following binding to stalled 60S ribosomal subunits, NEMF mediates CAT tailing by recruiting alanine-charged tRNA to the A-site and directing the elongation of stalled nascent chains independently of mRNA or 40S subunits, leading to non-templated C-terminal alanine extensions (CAT tails). Mainly recruits alanine-charged tRNAs, but can also other amino acid-charged tRNAs. 1. Shao, S. et al. (2015) Molecular cell 57, 433-44. PMID: 25578875 2. Hickey, KL. et al. (2020) Molecular cell 79, 950-962.e6. PMID: 32726578 3. Udagawa, T. et al. (2021) Cell reports 34, 108599. PMID: 33406423 4. Thrun, A. et al. (2021) Molecular cell 81, 2112-2122.e7. PMID: 33909987 |
| 注意事項 | For research use only |
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