Human NFATC4 Recombinant Protein (N-His) (HA801012)

Name: Human NFATC4 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA801012
Price: 211 USD
Availability: InStock

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1mg

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Vista general

Número de catálogo	HA801012
Descripción	Recombinant Human NFATC4 Protein, N-His (HA801012) expressed in E. coli, spanning Pro396-Pro685. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q14934
Longitud de proteína	Pro396-Pro685
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	34.59 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Nuclear factor of activated T-cells, cytoplasmic 4; NF-ATc4; NFATc4; T-cell transcription factor NFAT3; NF-AT3; NFATC4; NFAT3
Fondo	Nuclear factor of activated T-cells, cytoplasmic 4 is a ~95 kDa protein. Ca(2+)-regulated transcription factor that is involved in several processes, including the development and function of the immune, cardiovascular, musculoskeletal, and nervous systems. Involved in T-cell activation, stimulating the transcription of cytokine genes, including that of IL2 and IL4. Along with NFATC3, involved in embryonic heart development. Following JAK/STAT signaling activation and as part of a complex with NFATC3 and STAT3, binds to the alpha-beta E4 promoter region of CRYAB and activates transcription in cardiomyocytes. Involved in mitochondrial energy metabolism required for cardiac morphogenesis and function. 1. Yang, T. et al. (2001) The Journal of biological chemistry 276, 39569-76. PMID: 11514544 2. Yang, TT. et al. (2002) Molecular and cellular biology 22, 3892-904. PMID: 11997522 3. Cho, YY. et al. (2007) The Journal of biological chemistry 282, 8380-92. PMID: 17213202 4. Yao, K. et al. (2007) Cancer research 67, 8725-35. PMID: 17875713 5. Qin, X. et al. (2008) Cellular and molecular life sciences : CMLS 65, 2752-62. PMID: 18668201 6. Mei, Z. et al. (2015) Neurochemical research 40, 829-36. PMID: 25663301 7. Hoey, T. et al. (1995) Immunity 2, 461-72. PMID: 7749981 8. Yang, TT. et al. (2008) Molecular and cellular biology 28, 3489-501. PMID: 18347059
Nota	For research use only

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human NFATC4 Protein, N-His

Referencias

Datasheet

Human NFATC4 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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